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(increase or decrease) when comparing one extraction method to the other. For instance, the relative abundance of the orders Burkholderiales and Neisseriales, both belonging to the class Betaproteobacteria, were higher and lower, respectively, in mechanically-lysed samples (Figure S1 and Table S2). Similarly, one Streptococcus OTU was present at a significantly higher proportion in samples treated
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e retinal pigment epithelium/choroid and trimmed into a square of ,12 mm2. The retina was then placed photoreceptor side down in a small-volume (0.1 ml) chamber. The chamber was mounted on a fixed-stage upright microscope (Nikon Eclipse E600FN), and the retina superfused at 1.5 ml/min with bicarbonate-buffered Ames medium supplemented with 2 mg/ml D-(+) glucose and equilibrated with 5 CO2/95 O2.