ided to recapitulate the experimental settings [36], using BMB in alkylation reactions of total tRNA from E. coli, which is known to contain at least 1 pseudouridine per tRNA. This condition set 1 is characterized by a slightly acidic pH of 6.5 and high content of DMSO as solvent (75 ). As a negative control without modified nucleotides, an in-vitro-transcript (IVT) of M.m. tRNAAsp was used [42].
centrations were measured using the BCA method.Transient Transfections and Luciferase Activity AssayHEK293T cells in 24-well plates were transiently transfected with 1.25 mg of total DNA (expression plasmids for 900 ng of PXR, 300 ng of luciferase reporter vector, and 50 ng of pRL-tk) using SuperFect Transfection Reagent from Qiagen (Valencia, CA). 12 h after transfection, cells were treated with
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od. Determination of the severity of liver inflammation is important to evaluate the prognosis of patients with NASH. To explore the clinical usefulness of sCD14 as a biomarker for liver inflammation, we investigated the diagnostic ability of serum sCD14 levels using multiple regression analysis and ROC curves. We found that serum sCD14 levels are independently associated with increased risk of se
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